실험실 소개
생화학 교실은 생화학 및 생화학실습을 강의하고 이에 관련된 연구 활동을 하고 있다. 생화학은 생명현상을 의화학적으로 이해하려는 학문이다. 생명현상의 근원은 생명체에 존재하는 분자들의 화학적 상호작용에 있다. 생화학을 이수하려면 동물의 체내에서 일어나는 화학반응에 대한 생화학적 지식이 있어야 한다. 생화학자들은 생명체를 구성하는 분자들이 어떠한 구조와 기능을 가지고 있는지, 세포 내의 분자들이 어떻게 형성되고 변환되며 상호작용하는지, 또 생명체가 어떻게 생명에 필요한 에너지를 얻게 되는지를 연구한다. 따라서 수의생화학은 수의학의 가장 기초가 되는 학문의 하나로 본과 1학년 두 학기 동안 기본적인 수의생화학 강좌를 제공하고 있으며 대학원에서 심층적인 강좌 및 생리활성물질의 대사 및 질병에 대한 다양한 연구를 수행하고 있다.
연구내용
- 생리활성물질과 질병대사와의 상관성
- IgY를 이용한 동물항병성 및 산업화
- 생명자원을 이용한 생체 내 방어기작 기전규명
- 세포사멸 작용의 기전과 단백질의 발현 차이 규명
- 생명자원의 Bio-banking
최근 연구내용
연구내용
- 생리 활성 물질을 이용한 종양세포 세포사멸 기작과 Proteomics를 이용한 단백질의 발현
- 종양세포의 치료후보물질 선발을 위한 Intracellular molecular system 연구
연구방법
- Cell line : Human gastric and liver cancer cells
- Pectolinarigenin, Scutellarein, Sinensetin
- Western blot, Flow cytometry(FACS)
- Proteomics를 이용한 Bio marker 선별
: 2-DE를 통해 생리활성물질을 처리한 gastric cancer cell에서 단백질을 확인하고,
주요 단백질들은 분리하여 발현 차이와 상관관계를 분석하였다.
교수님소개
김곤섭 교수- 055-772-2346
- 담당과목 수의생화학
학력 사항
- 경상대학교 수의과대학: 1984(수의학사)
- 서울대학교 대학원 수의학과 생화학전공: 1986(수의학석사)
- 서울대학교 대학원 수의학과 생화학전공: 1992(수의학박사)
경력 사항
- 동국대학교 의과대학 조교
- 아산생명과학연구원 실험동물연구실 선임연구원
- 경상대학교 수의대 전임강사, 조교수, 부교수, 교수
- University of Pennsylvania ,School of Veterinary Medicine, USA.연구원
- 경상대학교 수의대 학장보,학과장,대학원주임
- 경상대학교 학생부처장 및 신문방송사 주간
- 전국국공립대학신문방송사주간교수협의회장
- 경상대학교 수의대 학장
- 사단법인 대한수의학회 부회장
- 경상남도 환경분쟁조정위원
- 식품의약안전처 안전성평가 심의위원, 정책 자문위원
- 식품의약안전처 정책 자문위원
- 대구첨단의료복합단지 실험동물센터 자문위원
- 경남 바이오21센터 운영위원
- 한국실험동물학회 연구이사, 경상남북도/부산/울산 지회장
- 동물생리활성물질자원 은행장/동물거점센터장
- 한국실험동물협회 실험동물시설 점검 전문위원
- 경남도 정책지원 자문위원
- 민주평화통일자문회의 자문위원
- International Journal of Oncology 학술/편집위원
- 한국임상보건과학회 회장
- 사단법인 대한수의학회 부회장, 회장
- 경상대학교 생명과학연구원장
- 한국교원단체총연합회 등기이사
- 대한독성유전단백체학회 부회장
- 사단법인 대한수의학회 이사장
주요 연구 업적 (최근 5년 논문: 2017-2021)
| 논문명 | 게재지 | 게재 연도 |
|---|---|---|
A Network Pharmacological Approach to Reveal thePharmacological Targets and Its Associated BiologicalMechanisms of Prunetin‐5‐O‐Glucoside against Gastric Cancer | Cancers | 2021 |
Activated ERK Signaling Is One of the Major Hub Signals Related to the Acquisition of Radiotherapy‐Resistant MDAMB‐231 Breast Cancer Cells | International journal of molecular sciences | 2021 |
Apoptotic Effects of Anthocyanins from Vitis coignetiae Pulliat Are Enhanced by Augmented Enhancer of the RudimentaryHomolog (ERH) in Human Gastric Carcinoma MKN28 Cells | International journal of molecular sciences | 2021 |
Iridin Induces G2/M Phase Cell Cycle Arrest and ExtrinsicApoptotic Cell Death through PI3K/AKT Signaling Pathway inAGS Gastric Cancer Cells | Molecules | 2021 |
Transcriptome analysis of sinensetin-treated liver cancer cells guided by biological network analysis | Oncology Letters | 2021 |
Comparative proteomic profile of canine uterus with pyometra | Journal of veterinary medicine | 2021 |
Quantitative Proteomics Analysis for the Identification of Differential Protein Expression in Calf Muscles between Young and Old SD Rats Using Mass Spectrometry | ACS Omega | 2021 |
A computational approach with biological evaluation: combinatorial treatment of curcumin and exemestane synergistically regulates DDX3 expression in cancer cell lines | Biomolecules | 2020 |
Naringin Induces Lysosomal Permeabilization and Autophagy Cell Death in AGS Gastric Cancer Cells | AMERICAN JOURNAL OF CHINESE MEDICINE | 2020 |
Apigetrin induces extrinsic apoptosis, autophagy, and G2/Mphasecell cycle arrest through PI3K/AKT/mTOR pathway in AGS human gastric cancer cell | Journal of Nutritional Biochemistry | 2020 |
Compound Prunetin Induces Cell Death in Gastric Cancer Cell with Potent Anti‐Proliferative Properties: In Vitro Assay, Molecular Docking, Dynamics, and ADMET Studies | Biomolecules | 2020 |
Artemisia iwayomogi (Dowijigi) inhibits lipopolysaccharide\_ induced inflammation in RAW264.7 macrophages by suppressing the NF-κB signaling pathway | EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2020 |
Flavonoid-induced apoptotic cell death in human cancer cells and its mechanisms: a review | Journal of Biomedical and Translational Research | 2020 |
Functions of flavonoids in Korean native varieties of Artemisia : Artemisia annua L., Artemisia iwayomogi and Artemisia argyi H. | Journal of Biomedical and Translational Research | 2020 |
Polyphenols Extracted from Artemisia annua L. Exhibit Anti-Cancer Eects on Radio-Resistant MDA-MB-231 Human Breast Cancer Cells by Suppressing Stem Cell Phenotype, -Catenin, and MMP-9 | Molecules | 2020 |
anthocyanins derived from vitis coignetiae pulliat contributes anti-cancer effect by suppressing NF-kB pathways in hep3B human hepatocellular carcinoma cells and in vivo. | Molecules | 2020 |
Pretreatment of Anthocyanin from the Fruit of Vitis coignetiae Pulliat Acts as a Potent Inhibitor of TNF-α Effect by Inhibiting NF-kB-Regulated Genes in Human Breast Cancer Cells | Molecules | 2020 |
Sinensetin Induces Autophagic Cell Death through p53-Related AMPK/mTOR Signaling in Hepatocellular Carcinoma HepG2 Cells | Nutrients | 2020 |
Cellular Function of Annexin A1 Protein Mimetic Peptide Ac2-26 in Human Skin Keratinocytes HaCaTand Fibroblast Detroit 551 Cells | Nutrients | 2020 |
Comparative proteomic analysis uncovers potential biomarkers involved in the anticancer effect of Scutellarein in human gastric cancer cells | Oncology Reports | 2020 |
anthocyanins isolated from vitis coignetiae pulliat enhances cisplatin sensitivity in MCF-7 human breast cancer cells through inhibition of Akt and NF-kB activation | Molecules | 2020 |
Function of flavonoids on different types of programmed cell death and its mechanism: a review | The Journal of Biomedical Research | 2019 |
Polyphenol mixture of a native Korean variety of Artemisia argyi H. (Seomae mugwort) and its anti-inflammatory effects | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2019 |
Proteome Profiling of Membrane-Free Stem Cell Components byNano-LS/MS Analysis and Its Anti-Inflammatory Activity | Evidence-based Complementary and Alternative Medicine | 2019 |
Sinensetin regulates age-related sarcopenia in cultured primary thigh and calf muscle in cultured primary thigh and calf muscle | BMC Complementary and Alternative Medicine | 2019 |
Membrane-Free Stem Cell Components Inhibit Interleukin-1α-Stimulated Inflammation and Cartilage Degradation in vitro and in vivo: A Rat Model of Osteoarthritis | International Journal of Molecular Sciences | 2019 |
Morin enhances auranofin anticancer activity by up-regulation of DR4 and DR5 and modulation of Bcl-2 through reactive oxygen species generation in Hep3B human hepatocellular carcinoma cells | PHYTOTHERAPY RESEARCH | 2019 |
Therapeutic Effect of Rumex japonicus Houtt. on DNCB-Induced Atopic Dermatitis-Like Skin Lesions in Balb/c Mice and Human Keratinocyte HaCaT Cells | NUTRIENTS | 2019 |
Anti-inflammatory activities of sinensetin on LPS-stimulated L6 skeletal muscle cells | Journal of Biomedical and Translational Research | 2019 |
Scutellarein Induces Fas-Mediated Extrinsic Apoptosis and G2/M Cell Cycle Arrest in Hep3B Hepatocellular Carcinoma Cells | NUTRIENTS | 2019 |
Essential oil from Korean Chamaecyparis obtusa leaf ameliorates respiratory activity in Sprague‑Dawley rats and exhibits protection from NF-κB-induced inflammation in WI38 fibroblast cells | NTERNATIONAL JOURNAL OF MOlECULAR MEDICINE | 2019 |
Identification of a novel biomarker in tangeretin‑ induced cell death in AGS human gastric cancer cells | ONCOLOGY REPORTS | 2018 |
Pectolinarigenin Induced Cell Cycle Arrest, Autophagy, and Apoptosis in Gastric Cancer Cell via PI3K/AKT/mTOR Signaling Pathway. | Nutrients | 2018 |
LC-MS/MS profiling of polyphenolenriched leaf stem and root extracts of Korean Humulus japonicus Siebold Zucc and determination of their antioxidant effects | Biomedical Chromatography | 2018 |
Effect of Harvest Season on Polyphenol Profile and Antioxidant Activities of Seomae Mugwort (Korean Artemisia argyi H. Lé v. & Vaniot) | SDRP Journal of Food Science & Technology | 2018 |
Comparative Proteomic Profiling of Tumor-Associated Proteins in Human Gastric Cancer Cells Treated with Pectolinarigenin | Nutrients | 2018 |
Lonicera japonica Thunb. Induces caspasedependent apoptosis through death receptors and suppression of AKT in U937 | PHYTOTHERAPY RESEARCH | 2017 |
Inhibition of IAP’s and activation of p53 leads to caspase-dependent apoptosis in gastric cancer cells treated with scutellarein | Oncotarget | 2017 |
LC MS/MS characterization, anti-inflammatory effects and antioxidant activities of polyphenols from different tissus of Korean Petasites japonicus (Meowi) | Biomedical chromatography | 2017 |
Anthocyanins from the Fruit of Vitis Coignetiae Pulliat Inhibit TNF-Augmented Cancer Proliferation, Migration, and Invasion in A549 Cells | Asian Pac J Cancer Prev | 2017 |
Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array | MOLECULAR MEDICINE REPORTS | 2017 |
Korean Scutellaria baicalensis georgi flavonoid extract induces mitochondrially mediated apoptosis in human gastric cancer AGS cells | Oncology Letters | 2017 |
Flavone polyphenols dominate in Thymus schimperi Ronniger: LC-ESI-MS/MS characterization and study of anti-proliferative effects of plant extract on AGS and HepG2 cancer cells | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2017 |
Polyphenolic compounds from Korean Lonicera japonica Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells | Oncology Letters | 2017 |
Flavonoids: A new generation molecule to stimulate programmed cell deaths in cancer cells. | Jornal of Biomedical and Translational Research | 2017 |
Tetraarsenic hexoxide induces G2/M arrest, apoptosis, and autophagy via PI3K/Akt suppression and p38 MAPK activation in SW620 human colon cancer cells | Plos One | 2017 |
Dopamine D3 receptor-modulated neuroprotective effects of lisuride | Neuropharmacology | 2017 |
KoreanByungkyul-CitrusplatymammaHort.etTanaka flavonoidsinducescellcyclearrestandapoptosis,regulating MMPproteinexpressioninHep3Bhepatocellularcarcinomacells | INTERNATIONALJOURNALOFONCOLOGY | 2017 |