수의과대학 수의과대학

연구내용

• 아메바성 수막뇌염 백신을 위한 특이 유전자 및 벡터 시스템 개발
• 파울러자유아메바 감염에 있어서 inflammasome 유전자 탐색 및 기능 분석
• 병원성 자유생활아메바의 병인기전과 숙주 방어면역 연구
• 인플루엔자바이러스의 동시 감별이 가능한 현장용 진단스트립의 개발
• 실시간 진단 모니터링시스템 구축을 위한 말라리아 신속진단키트 개발

교수님소개

학력 사항

• 연세대학교 (보건학사)
• 아주대학교 (의학석사)
• 아주대학교 (이학박사)

경력 사항

• 아주대학교 의과대학 미생물학교실 조교 (2004.3~2008.2)
• 아주대학교 의과대학 미생물학교실 연구강사 (2008.3~2013.2)
• 아주대학교 의과대학 미생물학교실 연구조교수 (2013.3~2014.2)

주요연구업적 - SCI(E) 논문

• De Novo Transcriptome Profiling of Naegleria fowleri Trophozoites and Cysts via RNA Sequencing. Pathogens, 12:174, 2023.
• Exploring the Fibrin(ogen)olytic, Anticoagulant, and Antithrombotic Activities of Natural Cysteine Protease (Ficin) with the-Carrageenan-Induced Rat Tail Thrombosis Model. Nutrients, 14:3552, 2022.
• Specific Detection of Acanthamoeba species using Polyclonal Peptide Antibody Targeting the Periplasmic Binding Protein of A. castellanii. Korean Journal of Parasitology, 60(2):143-147, 2022.
• Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification. Scientific Reports, 11:4183, 2021.
• Cellular characterization of actin gene concerned with contact dependent mechanisms in Naegleria fowleri. Parasite Immunology. 41:e12631, 2019.
• Therapeutic Effect of Rumex japonicus Houtt. on DNCB-Induced Atopic Dermatitis-Like Skin Lesions in Balb/c Mice and Human Keratinocyte HaCaT Cells. Nutrients, 11:573, 2019.
• A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea. Korean Journal of Parasitology, 56(4):375-378, 2018.
• Production and characterization of monoclonal antibodies against cathepsin B and cathepsin B-Like proteins of Naegleria fowleri. Experimental Parasitology, 183:171-177, 2017.
• Efficient Liquid Media for Encystation of Pathogenic Free-Living Amoebae. Korean Journal of Parasitology, 55(3):233-238, 2017.
• Excretory and Secretory Proteins of Naegleria fowleri Induce Inflammatory Responses in BV-2 Microglial Cells. Journal of Eukaryotic Microbiology, 64:183–192, 2017.
• Histopathological findings in wild Nutrias (Myocastor coypus) with Capillaria hepatica infection. Japanese Society of Veterinary Science, 78(12): 1887–1891, 2016.
• NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri. Infection and Immunity, 84(9):2422–2428, 2016.
• Effective PCR-based detection of Naegleria fowleri from cultured sampleand PAM-developed mouse. European Journal of Protistology, 51:401–408, 2015.
• Characterization of a gut-associated asparaginyl endopeptidase of Clonorchis sinensis. Experimental Parasitology, 153:81–90, 2015.
• Protective immunity against Naegleria fowleri infection on mice immunized with the rNfa1 protein using mucosal adjuvants. Parasitology Research, 114:1377–1385, 2015.
• Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory–secretory proteins and their biochemical properties. Parasitology Research, 113:2765–2776, 2014.
• Effects of Xanthium stramarium and Psoralea corylifolia Extracts Combined with UVA1 Irradiation on the Cell Proliferation and TGF-β1 Expression of Keloid Fibroblasts. Annals of Dermatology, 25(3):304-309, 2013.
• Vaccination with lentiviral vector expressing the nfa1 gene confers a protective immune response to mice infected with Naegleria fowleri. Clinical and Vaccine Immunology, 20(7):1055-1060, 2013.
• IL-1β and IL-6 activate inflammatory responses of astrocytes against Naegleria fowleri infection via the modulation of MAPKs and AP-1. Parasite Immunology, 35(3-4):120-128, 2013.
• The immune response induced by DNA vaccine expressing nfa1 gene against Naegleria fowleri. Parasitology Research, 111(6):2377-2384, 2012.
• Functional roles of mannose-binding protein in the adhesion, cytotoxicity and phagocytosis of Acanthamoeba castellanii. Experimental Parasitology, 132(2):287-292, 2012.
• Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA. Parasitology Research, 111(4):1645-1650, 2012.
• Induction of interleukin-8 by Naegleria fowleri lysates requires activation of ERK in human astroglial cells. Parasitology Research, 111(2):587-592, 2012.
• Naegleria fowleri lysate induces strong cytopathic effects and pro-inflammatory cytokine release in rat microglial cells. Korean Journal of Parasitology, 49(3):285-290, 2011.
• Effects of immunization with the rNfa1 protein on experimental Naegleria fowleri-PAM mice. Parasitology Immunology, 33(7):382-389, 2011.
• The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri. Parasitology Research, 106(4):917-924, 2011.
• Immunodominant antigens in Naegleria fowleri excretory-secretory proteins were potential pathogenic factors, Parasitology Research, 105(6):1675-1681, 2009.
• Gene silencing of nfa1 affects the in vitro cytotoxicity of Naegleria fowleri in murine macrophages. Molecular and Biochemical Parasitology, 165(1):87-93, 2009.
• Contact-independent cell death of human microglial cells due to pathogenic Naegleria fowleri trophozoites. Korean Journal of Parasitology, 46(4):217-221, 2008.
• TRAIL induces MMP-9 expression via ERK activation in human astrocytoma cells. Biochemical and Biophysical Research Communications, 377(1):195-199, 2008.
• Effect of therapeutic chemical agents in vitro and on experimental meningoencephalitis due to Naegleria fowleri. Antimicrobial Agents and Chemotherapy, 52(11):4010-4016, 2008.
• Therapeutic effect of rokitamycin in vitro and on experimental meningoencephalitis due to Naegleria fowleri. International Journal of Antimicrobial Agents, 32(5):411-417, 2008.
• Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity. Parasitology Research, 103(2):313-317, 2008.
• Naegleria fowleri: nfa1 gene knock-down by double-stranded RNAs. Experimental Parasitology, 118(2):208-213, 2008.
• Production of Nfa1-specific monoclonal antibodies that influences the in vitro cytotoxicity of Naegleria fowleri trophozoites on microglial cells. Parasitology Research, 101(5):1191-1196, 2007.
• Molecular cloning and characterization of a cytosolic heat shock protein 70 from Naegleria fowleri. Parasitology Research, 100(5):1083-1089, 2007.
• Cytopathic changes and pro-inflammatory cytokines induced by Naegleria fowleri trophozoites in rat microglial cells and protective effects of an anti-Nfa1 antibody. Parasite Immunology, 27(12):453-459, 2005.
• Role of the Nfa1 protein in pathogenic Naegleria fowleri cocultured with CHO target cells. Clinical and Diagnostic Laboratory Immunology, 12(7):873-876, 2005.